Virus Titration Essay

971 Words Apr 5th, 2016 4 Pages
Tissue culture, is the removal of cells from an animal or a plant, and growing the cells in a favourable artificial condition (Wiley, J.M et al, 2013). There are three types of cell culture: primary, this is cultivating the cells away from the parental tissue, secondary, which is when a primary culture is sub-cultured and cell lines, which can be continuous or finite depending on the life span of the culture. Virus titrations are used to estimate the virus concentration; it is a viral quantification technique. When detecting the virus, the cytopathic effect is looked at, whether there is lysis of the cells, vacuolation, formation of syncytia and the presence of inclusion bodies. TCID50, is the measure of the infectious
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The log of virus dilution that infected 50% of inoculated cell cultures. ID50 = 10-7.8

The Reed Muench method was used to obtain the results. The Reed-Muench method was used to determine the 50% endpoint in this experiment. From Table 1 the 50% end point can be calculated, quantifying the concentration of the virus required to produce a cytopathic effect in 50% of inoculated tissue culture cells.
Table 1 shows that the 50% end point lies between the log virus dilutions 10-7 and 10-8. From the results obtained, it can be seen that the 50% endpoint lies between the log virus dilutions 10-7 and 10-8. From this, the proportionate distance was able to be calculated. It was calculated by: (% positive above 50% -50%)/ (% positive above 50%) (% positive above 50%), the result obtained from this was 0.814 as the proportionate distance. This result, allowed the logID50 to be calculated, which was 10-7.8. This is the infective dose, which is the dose of the virus that will infect 50% of the cultures inoculated. The results in table 1 show that with the highest virus dilution, infected 100% of the test units, from the log virus dilutions 10-1 to 10-5, 100% of test units were infected. However, as the virus concentration decreased, the percentage of infected cells also decreased. As with the lowest log virus dilution of 10-10, the percentage of infected test units was 7%. Figure 1, suggests that the lower log virus dilution the smaller the number of inoculated

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