Essay about The Methods Of Gel Electrophoresis

1612 Words Dec 7th, 2015 7 Pages
Gel electrophoresis is a method used for separation and analysis of molecules such as DNA, RNA, and proteins, based on their sizes and polarity. DNA (deoxyribonucleic acid) is a molecule that carries most of our genetic information, and possesses a negative charge. During gel electrophoresis, DNA fragments can migrate through the gel also known as agarose when placed in a powerful electrical field. The rate at which the DNA fragments will move through the gel depends on their relative size. Horizontal gel slabs are commonly used on conducting gel electrophoresis. The agarose gel is poured onto a plastic plat forming wells, and then DNA samples are placed in small wells. When samples are added to their relative wells, gel and plastic plate are put into an electrophoresis chamber, and buffer is added. After the electrical current is turned on, DNA samples start to migrate. With staining process the DNA samples appear as series of bands spread from one end to the other of the gel.
Sickle cell anemia is an autosomal disease that is passed on from generation to generation. Sickle cell disease is a blood disease that is cause by abnormal hemoglobin. Hemoglobin is a protein that is present in red blood cells; it carries oxygen to cells in the body. Individuals who suffer from sickle-cell anemia, transport of oxygen is compromised due to the nucleotide mutation. The substitution of polar side chain amino acid Glutamic acid for non-polar side chain amino acid Valine results in the…

Related Documents