The Effect Of Control Plasmid And Plasmid Lux Essay
The effect of Control Plasmid and Plasmid Lux in Bacterial Transformation
31 October 2014
TA: Reinier Llorca
According to The General Biology I Lab manual, involved in Gene expression are two complex cellular pathways, each being subjected to regulation (Alberte et al., 2012). The first pathway is the synthesis of an RNA molecule from a DNA strand. This process is called transcription. When the RNA molecule exits the nucleus, it can be translated into a polypeptide sequence. This process is called translation. With some adjustments, these pathways happen in both prokaryotes and eukaryotes. Plasmids played a major role in this experiment. Control plasmid and Lux plasmid were added to bacteria on an agar plate, some with ampicillin, and some without. They were evenly spread across the agar plate and incubated at 37˚C for 7 days. I expected the agar plates with plasmid didn’t matter which plasmid would have colonial growth or no growth at all. I expected everything else to have lawn growth. According to my results, my predictions were not supported. I received lawn growth from all of my agar plates. Because of these results, it became known that errors were made during this experiment. All in all, the experiment taught me that bacteria can be easily manipulated by anything including plasmids which are tiny molecules.