Enzymes Lab Report Essay example

1671 Words Jul 25th, 2011 7 Pages
Enzymes Lab Report
Inroduction
In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions. It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate. Activation energy is the minimum amount of energy needed for a chemical reaction to occur, yielding products from a given set of reactants. (Unit 7: Enzymes lab) Products are results of the an enzyme cleaving to a specific substrate, by means of an induced fit. The induced fit is located at the active site of the enzyme or region of the enzyme where the substrate is bound. The substrate is the reactant
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Following that, a clean plastic pipette was used to add 1 drop of concentrated NaOH tho the basic tube, and was then tested with pH paper to verify that the pH was 12. Once the pH had been attained for each tube, lactase was added to bring the solution up to the 1.0 line of the tube. All tubes were then placed in a 40 degrees C water bath and incubated for 10 minutes. After 10 minutes, a glucose strip was placed in each tube for one second and removed to compare the coloration of the strip to the chart provided and the amount of glucose was determined in mg/dL. (Unit 7: Enzymes lab)
Specificity experiment One microfuge tube was labelled “Lactose” and another tube was labelled “Maltose”. Then, a clean plastic pipette was used to add milk up to the 0.5 line of the lactose and also a clean pipette was used to do the same in the maltose tube. Next, a clean plastic pipette was used to ad lactase to each tube until the level of mixture in each tube came up to the 1.0 line. Both tubes were then placed in a 40 degrees C bath and incubated for 10 minutes. After 10 minutes a glucose strip was placed in each tube for one second, removed, and allowed to sit on the bench top for one minute. At the end of one minute the strip coloration was compared to the chart provided and the amount of glucose was determined in mg/dL. (Unit 7: Enzymes lab)
Cofactor experiment One microfuge tube was labelled “Control” and another one “EDTA”. 0.5 M EDTA

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